目录产品 » CA125/MUC16[Biotin], His & Avi, Human
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CA125/MUC16[Biotin], His & Avi, Human

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
￥5800
Z04422-100

产品简介
Species Human
Protein Construction
CA125/MUC16 (Gly12660-Met12923)
Accession # Q8WXI7		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human MSLN, hFc Tag at 5μg/ml (100μl/well) on the plate can bind CA125/MUC16[Biotin], His & Avi, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 31.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 52-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CA125/MUC16[Biotin], His & Avi, Human

Immobilized Human MSLN, hFc Tag at 5 μg/ml(100 μl/well) on the plate. Dose response curve for CA125/MUC16[Biotin], His & Avi, Human, His Tag with the EC50 of 0.10 μg/ml determined by ELISA. »

CA125/MUC16[Biotin], His & Avi, Human

CA125/MUC16[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »

CA125/MUC16[Biotin], His & Avi, Human

The purity of CA125/MUC16[Biotin], His & Avi, Human is greater than 90% as determined by SEC-HPLC. »

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技术背景
Target Background MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Synonyms CA125; CA-125; CA125MUC-16; FLJ14303; MUC16
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.