Immobilized B7-2/CD86[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 1.7 ng/ml determined by ELISA.

B7-2/CD86[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-2/CD86[Biotin], His & Avi, Human

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

产品编号	Z04407
规格	100 μg 500 μg 大单询价
数量
价格	￥5800


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产品简介

Species

Human

Protein Construction

B7-2/CD86 (Leu26-Pro247) Accession # P42081-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. ImmobilizedB7-2/CD86[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human CTLA4, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

28.2 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

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