| ￥5800 | |
| Z04391-100 | |
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| Species | Human/Cynomolgus | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human Activin A, No Tag at 5μg/ml (100μl/well) on the plate can bind Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 16.56 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 33-43 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human Activin A, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus, His-Avi Tag with the EC50 of 47.7 ng/ml determined by ELISA. »
The purity of Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus is greater than 95% as determined by SEC-HPLC. »
Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). »
Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus, His Tag captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). »
Activin RIIB/ACVR2B[Biotin], His & Avi, Human/Cynomolgus, His Tag immobilized on CM5 Chip can bind Anti-Activin RIIB Antibody, hFc Tag with an affinity constant of 6.44 pM as determined in SPR assay (Biacore T200). »
| Target Background | ActRIIB (activin receptor type-2B) is an activin receptor subtype constitutively expressed in the whole body, playing a role in cellular proliferation, differentiation, and metabolism. For its various physiological activities, ActRIIB interacts with activin and multiple other ligands including myostatin (MSTN), growth differentiation factor 11 (GDF11), and bone morphogenetic protein 9 (BMP9). |
| Synonyms | Activin receptor type-2B; ACVR2B; Activin RIIB |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
