| ￥5800 | |
| Z04359-100 | |
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| Species | Cynomolgus | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized AntiBTN3A1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate can bind BTN3A1/CD277[Biotin], His & Avi, Cynomolgus. Test result was comparable to standard batch | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 26.5 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for BTN3A1/CD277[Biotin], His & Avi, Cynomolgus, His Tag with the EC50 of 2.1 ng/ml determined by ELISA. »
The purity of BTN3A1/CD277[Biotin], His & Avi, Cynomolgus is greater than 95% as determined by SEC-HPLC. »
BTN3A1/CD277[Biotin], His & Avi, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg). |
| Synonyms | CD277; BTN3A1; BTF5; BT3.1; BTN3.1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
