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CD47 hFc Chimera, Rat

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
￥2600
Z04353-100

产品简介
Species Rat
Protein Construction
CD47 (Gln19-Lys140)
Accession # P97829-1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse SIRP alpha, His Tag at 5μg/ml (100μl/well) on the plate can bind CD47 hFc Chimera, Rat. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 40.40 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CD47 HFc Chimera, Rat

Immobilized Mouse SIRP alpha, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for CD47 hFc Chimera, Rat, hFc Tag with the EC50 of 47.6 ng/ml determined by ELISA. »

CD47 HFc Chimera, Rat

The purity of CD47 hFc Chimera, Rat is greater than 95% as determined by SEC-HPLC. »

CD47 HFc Chimera, Rat

CD47 hFc Chimera, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.