| Species |
Mouse |
| Protein Construction |
CD27/TNFRSF7 (Pro24-Arg182)
Accession # P41272-1 |
mFc (IgG1) |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD27/TNFRSF7 mFc Chimera, Mouse at 2μg/ml (100μl/Well) on the plate can bind Mouse CD27 Ligand, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
44.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized CD27/TNFRSF7 mFc Chimera, Mouse, mFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.2 ng/ml determined by ELISA.
The purity of CD27/TNFRSF7 mFc Chimera, Mouse is greater than 95% as determined by SEC-HPLC.
CD27/TNFRSF7 mFc Chimera, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. |
| Synonyms |
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
