Immobilized CD27/TNFRSF7, His, Mouse, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA.

The purity of CD27/TNFRSF7, His, Mouse is greater than 95% as determined by SEC-HPLC

CD27/TNFRSF7, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD27/TNFRSF7, His, Mouse

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

产品编号	Z04307
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


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产品简介

Species

Mouse

Protein Construction

CD27/TNFRSF7 (Pro24-Arg182) Accession # P41272	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized CD27/TNFRSF7, His, Mouse at 1μg/ml (100μl/well) on the plate can bind Mouse CD27 Ligand, hFc Tag. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

19.40 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized CD27/TNFRSF7, His, Mouse, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA.

The purity of CD27/TNFRSF7, His, Mouse is greater than 95% as determined by SEC-HPLC

CD27/TNFRSF7, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Synonyms	CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.