| ￥3000 | |
| Z04279-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized NKG2D/CD314, His & Avi, Human at 1μg/ml (100μl/well) on the plate can bind AntiNKG2D Antibody, hFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 19.0 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 36-38 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized NKG2D/CD314, His & Avi, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA. »
NKG2D/CD314, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. »
NKG2D/CD314 hFc Chimera, Flag, Human, His Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. »
Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind NKG2D/CD314 hFc Chimera, Flag, Human , His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). »
| Target Background | NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. |
| Synonyms | CD314; D12S2489E; KLR; NKG2-D; NKG2D |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
