| ￥3000 | |
| Z04278-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human MICA, His Tag at 5μg/ml (100μl/Well) on the plate can bind Human NKG2D/CD314 hFc Chimera, Flag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 43.4 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human MICA, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag with the EC50 of 0.94 μg/ml determined by ELISA. »
The purity of NKG2D/CD314 hFc Chimera, Flag, Human is greater than 95% as determined by SEC-HPLC. »
NKG2D/CD314 hFc Chimera, Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Serial dilutions of Anti- NKG2D Antibody were added into Biotinylated Human ULBP-2, His Tag : NKG2D/CD314 hFc Chimera, Flag, Human, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 45.2 ng/ml. »
| Target Background | NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. |
| Synonyms | CD314; D12S2489E; KLR; NKG2-D; NKG2D |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
