| Species |
Human |
| Protein Construction |
IL-17R alpha/CD217 (Leu33-Trp320)
Accession # Q96F46-1 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per ug by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized IL-17R alpha/CD217, His, Human at 5μg/ml (100μl/well) on the plate can bind Biotinylated Human IL17A, His Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
34.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. |
| Synonyms |
CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
