Fc gamma RIIB/CD32b, His & Avi, Human

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

价格	￥2600 在线订购包邮(港澳台除外)
产品编号	Z04251-100
规格	100 μg 500 μg 大单询价
数量
参考货期


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产品简介图例技术背景

产品简介

Species

Human

Protein Construction

Fc gamma RIIB/CD32b (Ala46-Pro217) Accession # P31994-1	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIB/CD32b, His & Avi, Human in SPR assay (Biacore T200). Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

22.5 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 32-37 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

图例

The purity of Fc gamma RIIB/CD32b, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Fc gamma RIIB/CD32b, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIB/CD32b, His & Avi, Human, His Tag with an affinity constant of 3.46 μM as determined in SPR assay (Biacore T200). »

Fc gamma RIIB/CD32b, His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 16.72 μM as determined in SPR assay (Biacore T200) (QC Test). »

技术背景

Target Background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Synonyms	Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A; FcγRIIB; Fc γRIIB; Fcγ RIIB; Fc γ RIIB

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.