Immobilized Human CD30, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for CD30 Ligand/TNFSF8 mFc Chimera, Human, mFc Tag with the EC50 of 8.5 ng/ml determined by ELISA.

The purity of CD30 Ligand/TNFSF8 mFc Chimera, HumanCD30 Ligand/TNFSF8 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

CD30 Ligand/TNFSF8 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD30 Ligand/TNFSF8 mFc Chimera, Human

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

产品编号	Z04186
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


联系我们

产品简介

Species

Human

Protein Construction

mFc (IgG2a）	CD30 Ligand/TNFSF8 (Gln63-Asp234) Accession # P32971-1
N-term	C-term

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD30, His Tag at 0.5μg/ml (100μl/well) on the plate can bind CD30 Ligand/TNFSF8 mFc Chimera, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

46.2 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized Human CD30, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for CD30 Ligand/TNFSF8 mFc Chimera, Human, mFc Tag with the EC50 of 8.5 ng/ml determined by ELISA.

The purity of CD30 Ligand/TNFSF8 mFc Chimera, HumanCD30 Ligand/TNFSF8 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

CD30 Ligand/TNFSF8 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Synonyms	CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.