| ￥3500 | |
| Z04186-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD30, His Tag at 0.5μg/ml (100μl/well) on the plate can bind CD30 Ligand/TNFSF8 mFc Chimera, Human. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 46.2 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human CD30, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for CD30 Ligand/TNFSF8 mFc Chimera, Human, mFc Tag with the EC50 of 8.5 ng/ml determined by ELISA. »
The purity of CD30 Ligand/TNFSF8 mFc Chimera, HumanCD30 Ligand/TNFSF8 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
CD30 Ligand/TNFSF8 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. |
| Synonyms | CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
