Immobilized Anti-IL-2 R alpha Antibody, hFc Tag at 0.2 μg/ml (100 μL/well) on the plate. Dose response curve for IL-2 R alpha/CD25[Biotin], His & Avi, Human, His Tag with the EC50 of 12.4 ng/ml determined by ELISA.

The purity of IL-2 R alpha/CD25[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

IL-2 R alpha/CD25[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-2 R alpha/CD25[Biotin], His & Avi, Human

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

产品编号	Z04096
规格	100 μg 500 μg 大单询价
数量
价格	￥5800


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产品简介

Species

Human

Protein Construction

IL-2 R alpha/CD25 (Glu22-Cys213) Accession # P01589	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized AntiCD25 Antibody, hFc Tag at 0.2μg/ml (100μl/well) on the plate can bind IL-2 R alpha/CD25[Biotin], His & Avi, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

24.6 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of IL-2 R alpha/CD25[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

IL-2 R alpha/CD25[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Synonyms	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

IL-2 R alpha/CD25[Biotin], His & Avi, Human

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