目录产品 » CD30/TNFRSF8[Biotin], His & Avi, Human
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CD30/TNFRSF8[Biotin], His & Avi, Human

The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
￥5800
Z04063-100

产品简介
Species Human
Protein Construction
CD30/TNFRSF8 (Phe19-Lys379)
Accession # P28908-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate can bind CD30/TNFRSF8[Biotin], His & Avi, Human. Test result was comparable to standard batch
Expression System HEK293
Theoretical Molecular Weight 41.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CD30/TNFRSF8[Biotin], His & Avi, Human

Immobilized Human CD30 Ligand, His Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for CD30/TNFRSF8[Biotin], His & Avi, Human, His Tag with the EC50 of 17.5 ng/ml determined by ELISA. »

CD30/TNFRSF8[Biotin], His & Avi, Human

The purity of CD30/TNFRSF8[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

CD30/TNFRSF8[Biotin], His & Avi, Human

CD30/TNFRSF8[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

CD30/TNFRSF8[Biotin], His & Avi, Human

Immobilized Anti-CD30 Antibody, hFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for CD30/TNFRSF8[Biotin], His & Avi, Human, His Tag with the EC50 of 10.8 ng/ml determined by ELISA. »

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技术背景
Target Background The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Synonyms CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.