Immobilized Human CD27 Ligand (Trimer), His Tag at 5 μg/ml(100 μl/well) on the plate. Dose response curve for CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 0.14 μg/ml determined by ELISA.

The purity of CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

产品编号	Z04062
规格	100 μg 500 μg 大单询价
数量
价格	￥5800


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产品简介

Species

Human

Protein Construction

CD27/TNFRSF7 (Thr21-Ile192) Accession # P26842	hFc	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD27 Ligand (Trimer), His Tag at 5μg/ml (100μl/well) on the plate can bind CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

47.01 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC.

CD27/TNFRSF7 hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Synonyms	CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.