Species |
Human |
Protein Construction |
CD4/LEU3 (Lys26-Trp390) Accession # P01730 |
His |
Avi |
N-term |
|
C-term |
|
Conjugate |
Biotin |
Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Anti-CD4 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate can bind CD4/LEU3 [Biotin], His & Avi, Human. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
43.6 kDa |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS,(pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![CD4/LEU3 [Biotin], His & Avi, Human CD4/LEU3 [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z04015 ELISA.jpg&file=scm_files/productFile_notes/2025/04/25/Z04015 ELISA.jpg)
Immobilized Anti-CD4 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for CD4/LEU3[Biotin], His & Avi, Human, His Tag with the EC50 of 8.1 ng/ml determined by ELISA.
![CD4/LEU3 [Biotin], His & Avi, Human CD4/LEU3 [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z04015 HPLC.jpg&file=scm_files/productFile_notes/2025/04/25/Z04015 HPLC.jpg)
The purity of CD4/LEU3[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.
![CD4/LEU3 [Biotin], His & Avi, Human CD4/LEU3 [Biotin], His & Avi, Human](/product/documents/down?doc_name=Z04015 PAGE.png&file=scm_files/productFile_notes/2025/04/25/Z04015 PAGE.png)
CD4/LEU3[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Target Background |
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |
Synonyms |
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.