| Species |
Cynomolgus |
| Protein Construction |
CD3E/CD3 epsilon (Gln22-Asp117)
Accession # Q95LI5 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD3E/CD3 epsilon, His, Cynomolgus at 0.5μg/ml (100μl/well) on the plate can bind Anti-CD3 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
11.7 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 14-16 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. -80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Immobilized CD3E/CD3 epsilon, His, Cynomolgus, His Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.2 ng/ml determined by ELISA.
The purity of CD3E/CD3 epsilon, His, Cynomolgus is greater than 95% as determined by SEC-HPLC.
CD3E/CD3 epsilon, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
| Synonyms |
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
