Species |
Cynomolgus |
Protein Construction |
CD3E & CD3G [Gln22-Asp117 (CD3E) & Gln23-Thr113 (CD3G)] Accession # Q95LI5.2 (CD3E) & Q95LI7 (CD3G) |
His |
Flag |
N-term |
|
C-term |
|
Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3G, His & Flag, Cynomolgus at 1μg/ml (100μl/well) on the plate can bind Anti-CD3 Antibody, hFc Tag. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
11.93 kDa (CD3E) and 11.52 kDa (CD3G) |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 13-23 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS,(pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |

Immobilized CD3E&CD3G, His & Flag, Cynomolgus, His Tag at 1 μg/ml (100 μg/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 6.1 ng/ml determined by ELISA.

The purity of CD3E&CD3G, His & Flag, Cynomolgus is greater than 90% as determined by SEC-HPLC.

CD3E&CD3G, His & Flag, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Target Background |
T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Synonyms |
CD3; CD3G; CD3g; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.