Species |
Human |
Protein Construction |
CD3E & CD3D [Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)] Accession # P07766 (CD3E) & P04234 (CD3D) |
hFc |
Avi |
N-term |
|
C-term |
|
Conjugate |
Biotin |
Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
Endotoxin Level |
Less than 1EU per μg by the LAL method. |
Biological Activity |
Measured by its binding ability in a functional ELISA. ImmobilizedCD3E&CD3D hFc Chimera [Biotin], Avi, Human at 1μg/ml (100μl/well) on streptavidin (5μg/ml) precoated plate can bind OKT3, mFc Tag. Test result was comparable to standard batch. |
Expression System |
HEK293 |
Theoretical Molecular Weight |
40 kDa (CD3E) and 35.4 kDa (CD3D) |
Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS,(pH 7.4). |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![CD3E&CD3D HFc Chimera [Biotin], Avi, Human CD3E&CD3D HFc Chimera [Biotin], Avi, Human](/product/documents/down?doc_name=Z03995 ELISA.jpg&file=scm_files/productFile_notes/2025/04/25/Z03995 ELISA.jpg)
Immobilized CD3E&CD3D hFc Chimera [Biotin], Avi, Human, hFc Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 43.9 ng/ml determined by ELISA.
![CD3E&CD3D HFc Chimera [Biotin], Avi, Human CD3E&CD3D HFc Chimera [Biotin], Avi, Human](/product/documents/down?doc_name=Z03995 HPLC.jpg&file=scm_files/productFile_notes/2025/04/25/Z03995 HPLC.jpg)
The purity of CD3E&CD3D hFc Chimera [Biotin], Avi, Human was greater than 95% as determined by SEC-HPLC.
![CD3E&CD3D HFc Chimera [Biotin], Avi, Human CD3E&CD3D HFc Chimera [Biotin], Avi, Human](/product/documents/down?doc_name=Z03995 PAGE.png&file=scm_files/productFile_notes/2025/04/25/Z03995 PAGE.png)
CD3E&CD3D hFc Chimera [Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Target Background |
T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.