| ￥6600 | |
| Z03995-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. ImmobilizedCD3E&CD3D hFc Chimera [Biotin], Avi, Human at 1μg/ml (100μl/well) on streptavidin (5μg/ml) precoated plate can bind OKT3, mFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 40 kDa (CD3E) and 35.4 kDa (CD3D) | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized CD3E&CD3D hFc Chimera [Biotin], Avi, Human, hFc Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 43.9 ng/ml determined by ELISA. »
The purity of CD3E&CD3D hFc Chimera [Biotin], Avi, Human was greater than 95% as determined by SEC-HPLC. »
CD3E&CD3D hFc Chimera [Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Immobilized Anti-CD3 Anitibody, hFc Tag at 5 μg/ml(100 μl/well) on the plate. Dose response curve for CD3E&CD3D hFc Chimera [Biotin], Avi, Human, hFc Avi Tag with the EC50 of 0.12 μg/ml determined by ELISA. »
Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind CD3E&CD3D hFc Chimera [Biotin], Avi, Human, hFc-Avi Tag with an affinity constant of 0.17 µM as determined in SPR assay (Biacore T200). »
| Target Background | T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Synonyms | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
