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CD3E&CD3G, His, Human

T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
￥4100
Z03988-100

产品简介
Species Human
Protein Construction
CD3E & CD3G [Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)]
Accession # P07766 (CD3E) & P09693 (CD3G)		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3G, His, Human at 2μg/ml (100μl/well) on the plate can bind OKT3, mFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 17.9 kDa (CD3E) and 17.9 kDa (CD3G)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CD3E&CD3G, His, Human

Immobilized CD3E&CD3G, His, Human at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 77.2 ng/ml determined by ELISA. »

CD3E&CD3G, His, Human

The purity of CD3E&CD3G, His, Human is greater than 95% as determined by SEC-HPLC. »

CD3E&CD3G, His, Human

CD3E&CD3G, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

CD3E&CD3G, His, Human

Immobilized CD3E&CD3G, His, Human at 2 μg/ml(100μl/well) on the plate. Dose response curve for Anti-CD3 Antibody , hFc Tag with the EC50 of 37.5 ng/ml determined by ELISA. »

CD3E&CD3G, His, Human

CD3E&CD3G, His, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3; CD3G; CD3g; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.