目录产品 » EGFR/HER1[Biotin], His & Avi, Human
Manual
SDS
COAs

EGFR/HER1[Biotin], His & Avi, Human

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
￥5700
Z03920-100

产品简介
Species Human
Protein Construction
EGFR/HER1 (Leu25-Ser645)
Accession # P00533-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Immobilized EGFR/HER1[Biotin], His & Avi, Human (Cat.No.: Z03920) at 1 μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml) can bind Human EGF, hFc Tag
Expression System HEK293
Theoretical Molecular Weight 71.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4 .
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
返回

图例
EGFR/HER1[Biotin], His & Avi, Human

Immobilized EGFR/HER1[Biotin], His & Avi, Human at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human EGF, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA. »

EGFR/HER1[Biotin], His & Avi, Human

EGFR/HER1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

EGFR/HER1[Biotin], His & Avi, Human

The purity of EGFR/HER1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

EGFR/HER1[Biotin], His & Avi, Human

EGFR/HER1[Biotin], His & Avi, Human captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). »

<
>

技术背景
Target Background The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
返回

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.