| ￥5700 | |
| Z03884-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Immobilized Anti-B7-H3 Antibody, hFc Tag at 1 μg/ml on the plate can B7-H3/CD276 hFc Chimera[Biotin], Avi, Human (Cat.No.: Z03884) | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 51.06 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. | ||||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O more than 100 μg/ml. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
B7-H3/CD276 hFc Chimera[Biotin], Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
The purity of B7-H3/CD276 hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC. »
Immobilized Anti-B7-H3 Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for B7-H3/CD276 hFc Chimera[Biotin], Avi, Human with the EC50 of 23.4 ng/ml determined by ELISA. »
| Target Background | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| Synonyms | B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
