Immobilized B7-H3/CD276[Biotin], His & Avi, Human at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.

The purity of B7-H3/CD276[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-H3/CD276[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

B7-H3/CD276[Biotin], His & Avi, Human

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

产品编号	Z03882
规格	100 μg 500 μg 大单询价
数量
价格	￥5700


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产品简介

Species

Human

Protein Construction

B7-H3/CD276 (Leu29-Pro245) Accession # Q5ZPR3-2	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Immobilized B7-H3/CD276[Biotin], His & Avi, Human (Cat.No.: Z03882) at 0.5 μg/ml (100μl/Well) on the streptavidin precoated plate can bind Anti-B7-H3 Antibody, hFc Tag .

Expression System

HEK293

Theoretical Molecular Weight

26.49 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of B7-H3/CD276[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

B7-H3/CD276[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Synonyms	B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.