Immobilized CEACAM-5/CD66e[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA.

The purity of CEACAM-5/CD66e[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CEACAM-5/CD66e[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

CEACAM-5/CD66e[Biotin], His & Avi, Human

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

产品编号	Z03834
规格	100 μg 500 μg 大单询价
数量
价格	￥5800


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产品简介

Species

Human

Protein Construction

CEACAM-5/CD66e (Lys35-Ala685) Accession # P06731-1	His	Avi
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Immobilized CEACAM-5/CD66e[Biotin], His & Avi, Human (Cat.No.: Z03834) at 2 μg/ml (100μl/Well) on the plate can bind Anti-CEACAM5 Antibody.

Expression System

HEK293

Theoretical Molecular Weight

74.23 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 110-150 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

The purity of CEACAM-5/CD66e[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC.

CEACAM-5/CD66e[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

技术背景

Target Background	CEACAM5 (Carcinoembryonic antigen-related cell adhesion molecule 5), is also known as CD66e (Cluster of Differentiation 66e). The overproduction of CEACAM5 could enrich for an epithelial gene expression pattern and facilitate tumor outgrowth at metastatic sites. CEACAM5 is often used as a synonym for many types of malignancies, such as colorectal cancer and non-small-cell lung cancer.
Synonyms	CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.