| ￥3500 | |
| Z03824-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Immobilized Nectin-4 hFc Chimera, Human (Cat.No.: Z03824) at 5 μg/ml (100μl/Well) can bind Human Nectin-1, His Tag. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 61.1 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Nectin-4 hFc Chimera, Human, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.2 μg/ml determined by ELISA. »
The purity of Nectin-4 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
Nectin-4 hFc Chimera, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »
Nectin-4 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). »
| Target Background | Nectin-4 is a tumor-associated antigen. It is probably involved in cell adhesion through trans-homophilic and -heterophilic interactions. Nectin-4 is overexpressed in urothelial carcinoma and several other malignancies. It is a promising target for antibody-drug conjugates (ADCs). |
| Synonyms | EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
