| ￥4200 | |
| Z03816-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||||
| Protein Construction |
The protein is composed of CD3E and CD3D
|
||||||
| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Immobilized CD3E&CD3D, His, Human (Cat.No.: Z03816) at 2 μg/ml can bind Anti-CD3 Antibody (OKT3). | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 17.9 kDa for CD3E; 16.7 kDa for CD3D | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 28-38 kDa and 25 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA. »
The purity of CD3E&CD3D, His, Human was greater than 95% as determined by SEC-HPLC. »
CD3E&CD3D, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 39.3 ng/ml determined by ELISA. »
Immobilized CD3E&CD3D, His, Human at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 4.7 ng/ml determined by ELISA. »
Immobilized CD3E&CD3D, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody
CD3E&CD3D, His, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200). »
| Target Background | The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Synonyms | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
