目录产品 » CD3E&CD3D, His, Human
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CD3E&CD3D, His, Human

The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
￥4200 在线订购包邮(港澳台除外)
Z03816-100

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产品简介
Species Human
Protein Construction

The protein is composed of CD3E and CD3D

CD3E (Asp23-Asp126)
Accession # P07766		 His tag
CD3D (Phe22-Ala105)
Accession # P04234		 His tag
N-term C-term
Purity > 95% as determined by SDS-PAGE
> 95% as determined by HPLC
Endotoxin Level < 1 EU/μg by gel clotting method
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3D, His, Human at 1.7μg/ml (100μl/Well) on the plate can bind OKT3, mFc Tag. Test result was comparable to standard batch.
Expression System CHO
Theoretical Molecular Weight 18.0 kDa for CD3E; 16.1 kDa for CD3D
Apparent Molecular Weight Due to glycosylation, the protein migrates to 18-25 kDa and16-35 kDa under reduced (R) condition based on SDS-PAGE result
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the lyophilized product remains stable for 6 months at -20℃ or below as supplied from the date of receipt. -80℃ for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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图例
CD3E&CD3D, His, Human

Immobilized Human CD3E&CD3D, His Tag at 1.7 μg/mL (100 μL/Well) on the plate. A Dose response curve in ELISA yielded an EC50 of 96.2 ng/mL for OKT3, hFc Tag. »

CD3E&CD3D, His, Human

The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC. »

CD3E&CD3D, His, Human

CD3E&CD3D, His, Human on SDS-PAGE under reducing condition. The purity is greater than 95%. »

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技术背景
Target Background The T-cell surface glycoproteins CD3E and CD3D, also called CD3 epsilon and CD3 delta chain, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate the T-cell receptor (TCR), TCR signals are transmitted by the CD3 chains CD3D, CD3E, CD3G, and CD3Z. All CD3 chains have immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.