| ￥2700 | |
| Z03814-100 | |
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| Species | Human | |
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | |
| Endotoxin Level | Less than 0.1EU per μg by the LAL method. | |
| Biological Activity | Immobilized Activin A, Human (Cat.No.: Z03814) at 1 μg/ml can bind Human Activin RIIB, His Tag. Activin A, Human (Cat.No.: Z03814) can inhibit proliferation of MPC-11 cells. | |
| Expression System | HEK293 | |
| Theoretical Molecular Weight | 12.97 kDa | |
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22 μm filtered solution in 4 mM HCI. | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI. | |
| Storage & Stability | Upon receiving, the lyophilized product remains stable up to 6 months at -20 °C or below as supplied from date of receipt.-80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 18.6 ng/ml determined by ELISA. »
Immobilized Activin A, Human at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 2.1 ng/ml determined by ELISA. »
Immobilized Activin A, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. »
Biotinylated Human Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Activin A, Human, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). »
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL. »
| Target Background | Activin-A, a member of the TGF-β superfamily, has fundamental roles in embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation, and tissue fibrosis. It signals through two type I and two type II receptors, activating kinase activity, phosphorylating SMAD2 and 3 intracellular signaling mediators, forming a complex with SMAD4, and translocating to the nucleus to regulate gene expression. |
| Synonyms | INHBA; Activin A; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
