| Species |
Monkeypox |
| Protein Construction |
A29L protein (Met1-Glu110)
Accession # Q77HM6 |
His |
| N-term |
C-term |
|
| Purity |
≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
13.4 kDa |
| Apparent Molecular Weight |
13.4 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 2.5% D-(+)-Trehalose Anhydrous, 2.5% D-Mannitol, PBS, pH 7.4. |
| Reconstitution |
Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 °C. |
Lane 1: 2 µg of Monkeypox A29L protein, His Tag, reducing (R)
The purity is greater than 95% as analyzed by SDS-PAGE
| Target Background |
Monkeypox virus is an enveloped double-stranded DNA virus that belongs to the Orthopoxvirus genus of the Poxviridae family. It is a viral zoonosis that causes similar symptoms to smallpox, although it is clinically less severe. Vaccination against smallpox was demonstrated through several observational studies to be about 85% effective in preventing monkeypox. Since early May 2022, more than 16,000 cases of monkeypox have been reported from countries where the disease is not endemic, and continue to be reported in several endemic countries, WHO has claimed monkeypox as a "Public Health Emergency of International Concern (PHEIC)". The A29L protein from Monkeypox binds to cell surface heparin to promote fusion of viral membrane with host plasma membrane, it is a potential target for developing diagnosis and vaccine. |
| Synonyms |
A29 protein; MPXV A29L; MPXV A29 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
