目录产品 » CA125/MUC16, His & Avi, Human
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CA125/MUC16, His & Avi, Human

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
￥2800
Z03650-100

产品简介
Species Human
Protein Construction
CA125/MUC16 (Gly12660-Met12923)
Accession # Q8WXI7		 Poly-His Avi
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level ≤ 1 EU/μg of protein by LAL method
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human CA125, His & Avi Tag at 5μg/ml (100μl/Well) on the plate can bind Human MSLN, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 31.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
CA125/MUC16, His & Avi, Human

Immobilized Human CA125, His & Avi Tag at 5 μg/ml(100 μl/Well). Dose response curve for Human MSLN with the EC50 of 3.78 μg/ml determined by ELISA. »

CA125/MUC16, His & Avi, Human

Recombinant MUC16 Protein on SDS-PAGE under reducing conditions. The purity is greater than 95%. »

CA125/MUC16, His & Avi, Human

The purity of Recombinant Human Muc16/CA125 Protein is greater than 95% as determined by SEC-HPLC. »

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技术背景
Target Background MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Synonyms CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.