| ￥1760 | |
| Z03643-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | ≤ 1 EU/μg of protein by LAL method | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Siglec-2/CD22, hFc, Human at 2μg/ml (100μl/well) on the plate can bind Biotinylated AntiSiglec2 Antibody, hFc Tag. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 101.9 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 120-130 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human Siglec2 at 0.5 μg/ml (100 μl/Well). Dose response curve for Biotinylated Anti-Siglec2 Ab with the EC50 of 0.2 μg/ml determined by ELISA. »
Recombinant Siglec-2/CD22 on SDS-PAGE under reducing conditions. The purity is greater than 95%. »
The purity of Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC »
Immobilized Siglec-2/CD22, hFc, Human, hFc Tag at 2 μg/ml(100 μL/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 14.9 ng/ml determined by ELISA. »
The purity of Siglec-2/CD22, hFc, Human is greater than 95% as determined by SEC-HPLC »
Siglec-2/CD22, hFc, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
| Synonyms | CD22 molecule; CD22; Siglec2; Siglec-2;B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
