| ￥1600 | |
| Z03635-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | ≤ 1 EU/μg of protein by LAL method | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized EpCAM/Trop1, His, Human at 0.2μg/ml (100μl/well) on the plate can bind AntiEpCAM Antibody, hFc Tag. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 28.2 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4. | ||||
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O more than 100 μg/ml. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human EpCAM at 0.2 μg/ml(100 μl/Well). Dose response curve for Anti-Trop1 Ab with the EC50 of 8.7 ng/ml determined by ELISA. »
Recombinant EpCAM on SDS-PAGE under reducing conditions, the purity is greater than 95%. »
The purity of EpCAM is greater than 95% as determined by SEC-HPLC. »
| Target Background | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
| Synonyms | CD326 antigen; CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; ACSTD1; antigen identified by monoclonal AUA1; Cell surface glycoprotein Trop-1; DIAR5; Epithelial glycoprotein 314; Epithelial glycoprotein; gp40; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MOC31; TACST-1; TACSTD1; TROP1; Tumor-associated calcium signal transducer 1CO-17A |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
