Species |
Porcine |
Protein Construction |
IL-1β (Ala115- Pro267) Accession # P26889 |
|
Purity |
> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
Endotoxin Level |
< 1 EU/μg of protein by LAL method |
Biological Activity |
The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of 2.0 × 105 IU/mg. |
Expression System |
E. coli |
Theoretical Molecular Weight |
17.6 kDa |
Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, 3 % trehalose. |
Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Target Background |
Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and have similar but not identical biological properties. Recombinant porcine interleukin-1 beta is a 17 kDa protein containing 153 amino acid residues and it shares 63 % - 70 % a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL1β. |
Synonyms |
IL-1 beta |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.