|Synonyms||CD80; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7|
|Description||B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature human B7-1 consists of a 208 amino acid extracellular domain (ECD) with two immunoglobulin-like domains, a 21 amino acid transmembrane domain, and a 25 amino acid cytoplasmic domain. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and sCTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Recombinant Human B7-1(CD80) Fc Chimera produced in HEK293 cells is a polypeptide chain containing 443 amino acids with the C-terminal human IgG1 Fc fragment. A fully biologically active molecule; rhB7-1(CD80) a molecular mass of 70 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||Activity 1：Immobilized CTLA 4 Fc Chimera, Human(Cat.No.Z03373) at 2 μg/mL (100 μL/well) can bind biotinylated B7-1(CD80) Fc Chimera, Human with a linear range of 1.22-9.77 ng/mL.
Activity 2：Immobilized CD28 Fc Chimera, Human(Cat.No.Z03413) at 2 μg/mL (100 μL/well) can bind biotinylated B7-1(CD80) Fc Chimera, Human with a linear range of 12.2-390.6 ng/mL.
|Measured Molecular Weight||75-80 kDa; observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH₂O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant B7-1(CD80), Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution; Human B7-1(CD80) should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|