|Synonyms||Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI,IFN-gamma.|
|Description||Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers.
Recombinant human Interferon gamma (rhIFN-γ) produced in E. coli is a non‐glycosylated polypeptide chain of 144 amino acids. A fully biologically active molecule, rhIFN-γ has a molecular mass of 17 kDa analyzed by reducing SDS‐PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
|Biological Activity||ED50<0.05ng/ml, measured by cytotoxicity assay using HT‐29 cells.|
MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE
SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV
KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE
LSPAAKTGKR KRSQMLFRGR RASQ
|Measured Molecular Weight||17kDa, observed by reducing SDS‐PAGE.|
|Purity||> 95% as analyzed by reducing SDS‐PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<1 EU/μg, determined by LAL|
|Sequence Analysis||Gln24‐Gln166 (accession number:P01579). Expression construct with an N‐terminal Met.|
|Storage||Lyophilized recombinant human Interferon gamma (rhIFN‐γ) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.|