StrepCaptureXP-iFluor647

StrepCaptureXP is a proprietary affinity protein that specifically binds Strep-tagged proteins with high specificity and reliability. When conjugated with iFluor647, it enables sensitive fluorescence-based detection in applications such as immunofluorescence and flow cytometry.

价格	￥5920 在线订购包邮(港澳台除外)
产品编号	M01236-200
规格	200 μl 大单询价
数量
参考货期


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产品概述应用指导产品属性图例技术背景

产品概述

Specificity	Strep-tag® II (WSHPQFEK) and Twin-Strep-tag® (WSHPQFEKGGGSGGGSGGSAWSHPQFEK) .
Conjugate	iFluor647

应用指导

Optimal working concentrations should be determined by the end user for each specific application. The appropriate concentration may vary depending on factors such as target protein expression level, assay format, detection sensitivity, incubation conditions, and instrumentation. The suitability of this product for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Flow Cytometry	1:50-1:200

产品属性

Form	Liquid
Storage buffer	PBS (pH 7.2), containing 10 mg/mL BSA , 0.02% sodium azide and 50% glycerol.
Concentration	0.5 mg/ml
Storage Instructions	Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.

图例

Jurkat cells were analyzed by flow cytometry to evaluate the detection of CD3-binding Strep-tagged proteins using StrepCaptureXP-iFluor647.
Cells were incubated with Strep-tagged UCHT1 (anti-CD3 monoclonal antibody) or Twin Strep-tagged Blinatumomab (CD3/CD19 bispecific antibody), followed by detection with StrepCaptureXP-iFluor647(GenScript, M01236). A no-antibody control (StrepCaptureXP-iFluor647 only) was included to assess background staining. »

Human PBMCs were analyzed by flow cytometry.
1. FMO control was used to define background fluorescence.
2. Cells were stained with an APC-conjugated anti-CD45RA antibody.
3. Cells were stained with a Strep-tagged anti-CD45RA antibody, followed by detection with StrepCaptureXP-iFluor647(GenScript, M01236) . »

技术背景

Synonyms	StrepCaptureXP
Target Background	Strep-tag® II and Twin-Strep-tag® are widely used affinity tags for recombinant protein purification and detection. StrepCaptureXP is a proprietary affinity protein that specifically binds Strep-tagged proteins with high specificity and reliability. When conjugated with iFluor647, it enables sensitive fluorescence-based detection in applications such as immunofluorescence and flow cytometry.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.