StrepCaptureXP-HRP

StrepCaptureXP is a proprietary affinity protein that specifically binds Strep-tagged proteins with high specificity and reliability. When conjugated with horseradish peroxidase (HRP), it enables sensitive detection in applications such as Western blot and ELISA.

价格	￥5920 在线订购包邮(港澳台除外)
产品编号	M01235-200
规格	200 μl 大单询价
数量
参考货期


咨询产品问题

产品概述应用指导产品属性图例技术背景

产品概述

Specificity	Strep-tag® II (WSHPQFEK) and Twin-Strep-tag® (WSHPQFEKGGGSGGGSGGSAWSHPQFEK) .
Conjugate	HRP

应用指导

Optimal working concentrations should be determined by the end user for each specific application. The appropriate concentration may vary depending on factors such as target protein expression level, assay format, detection sensitivity, incubation conditions, and instrumentation. The suitability of this product for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1,000 - 1:5,000 Dilution
ELISA	1:2,000 - 1:10,000 Dilution

产品属性

Form	Liquid
Storage buffer	PBS (pH 7.2), containing 10 mg/mL BSA, 0.01% thimerosal and 50% glycerol.
Concentration	0.5 mg/ml
Storage Instructions	Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.

图例

4 different Strep-tagged proteins were captured by their corresponding antibodies coated at 2 μg/mL. Proteins were applied in a serial dilution starting from 1 μg/mL. Detection was performed using StrepCaptureXP-HRP (GenScript, M01235) or an HRP-conjugated reagent from Supplier A at 0.1 μg/mL. »

A Strep-tagged protein was captured by its corresponding antibody coated at 2 μg/mL. The protein was applied in a serial dilution starting from 1 μg/mL. Detection was performed using StrepCaptureXP-HRP (GenScript, M01235) at 0.1 μg/mL with incubation times of 5, 10, 15, 20, and 30 minutes, respectively. Comparable signal intensities were observed across the tested incubation times. »

Detection sensitivity of the same Strep-tagged proteins by Western blot was compared using StrepCaptureXP-HRP (GenScript, M01235) and an HRP-conjugated reagent from Supplier A at an equivalent concentration (0.125 μg/mL). »

Western blot analysis of 8 different Strep-tagged proteins was performed using StrepCaptureXP-HRP (GenScript, M01235) and an HRP-conjugated reagent from Supplier A at an equivalent concentration (0.125 μg/mL). »

Western blot analysis was performed using E. coli, yeast, and CHO-S whole cell lysates, as well as HEK293 cell culture supernatant lacking Strep-tagged proteins.
Detection was performed using StrepCaptureXP-HRP (GenScript, M01235) and an HRP-conjugated reagent from Supplier A at an equivalent concentration (0.125 μg/mL). Proteins containing Strep-tags at the N- or C-terminus were included as positive controls. »

技术背景

Synonyms	StrepCaptureXP
Target Background	Strep-tag® II and Twin-Strep-tag® are widely used affinity tags for recombinant protein purification and detection. StrepCaptureXP is a proprietary affinity protein that specifically binds Strep-tagged proteins with high specificity and reliability. When conjugated with horseradish peroxidase (HRP), it enables sensitive detection in applications such as Western blot and ELISA.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.