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GenLNP-CX175-CD7VHH-EGFP mRNA (m1Ψ)

GenLNP-CX175-CD7VHH-EGFP mRNA (m1Ψ) is a CD7-targeted LNP formulated with CX175 and loaded with eGFP mRNA (m1Ψ). Featuring CD7 VHH-mediated targeting, it enables efficient delivery to CD7+ immune cells for targeted transfection and mRNA delivery research.
￥4500 在线订购包邮(港澳台除外)
RP-A00083-50

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Description

The GenLNP-CX175-CD7VHH-EGFP mRNA (m1Ψ) is a CD7 VHH-decorated lipid nanoparticle (LNP) loaded with eGFP mRNA (m1Ψ). The LNP displays anti-human CD7 VHH antibody (CD7-VHH-hIgG1Fc (LALA)) on its surface to enhance targeting efficiency to CD7-positive cells. CD7 is highly expressed on the majority of mature T cells, NK cells, and their early precursors, making it an attractive target for selective delivery both in vitro and in vivo. The antibody is covalently conjugated to lipid molecules to achieve targeted delivery.  
 
This LNP features: 
- Formulation with CX175, a licensable LNP from GenScript, as the main ionizable lipid for efficient encapsulation and delivery 
- CD7 VHH-mediated targeting for enhanced delivery to CD7+ immune cells 
- eGFP mRNA (m1Ψ) payload with 100% substitution of U bases with N1-methyl-pseudouridine (m1Ψ) for enhanced protein expression and reduced immunogenicity 
- Optimized formulation for targeted mRNA delivery applications  
 
This product enables efficient delivery of eGFP mRNA to CD7+ cells and is ideal for targeted transfection optimization, immune cell engineering research, and targeted mRNA delivery studies.
产品属性
Form Liquid
Concentration 0.15mg/mL
Full mRNA length 980 nt
Full mRNA Molecular Weight 319792 Da
Storage buffer Tris-HCl/Sucrose
Storage condition Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.
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质控参数
Appearance Clear and free of foreign particles
Encapsulation Efficiency > 85%
Endotoxin < 4 EU/ml
Particle Size 65-165 nm
pH 7.4 ± 0.5​
Polydispersity Index < 0.2
Zeta Potential ±20 mV
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应用指导
1. LNP can be added directly to cells incubated in complete media  
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium  
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate  
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression  
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting
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图例
GenLNP-CX175-CD7VHH-EGFP MRNA (m1Ψ)

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GenLNP-CX175-CD7VHH-EGFP MRNA (m1Ψ)

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.