BCMA CAR mRNA (Cap1, m1Ψ)

BCMA CAR mRNA encodes a chimeric antigen receptor targeting malignant plasma cells. Features Cap1, X5 codon optimization, m1Ψ modification, and 100A tail. Enables transient, non-integrating CAR expression for multiple myeloma research.

价格	￥1500
产品编号	RP-A00080-0.2
规格	0.2 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The BCMA CAR mRNA encodes a chimeric antigen receptor (CAR) that specifically targets B-cell maturation antigen (BCMA) on malignant plasma cells. Upon transfection, this mRNA enables transient CAR expression in immune effector cells, allowing them to recognize and eliminate BCMA+ multiple myeloma cells. The CAR construct typically includes an anti-BCMA scFv, hinge and transmembrane regions, and intracellular signaling domains such as 4-1BB and CD3ζ for activation and persistence.

This mRNA features:
- A Cap1 structure with high capping efficiency for enhanced translation
- Codon optimization via GenScript X5 algorithm for improved protein expression
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) to enhance expression and reduce innate immune activation
- A 100A poly(A) tail to mimic mature mRNA

This BCMA CAR mRNA is ideal for generating CAR-expressing immune cells for research and preclinical evaluation of multiple myeloma therapies.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1738 nt
Full mRNA Molecular Weight	560055 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Jurkat cells were transfected with BCMA CAR mRNA formulated in lipid nanoparticles (LNPs) to enable efficient mRNA delivery and transient CAR expression. Following transfection, surface expression of the BCMA CAR on Jurkat cells was evaluated using a fluorescently labeled Fab antibody Alexa Fluor® 647-F(ab')2 Goat Anti-Mouse IgG, F(ab')2 specific to the CAR construct. This assay confirms successful mRNA translation and membrane localization of the BCMA CAR, validating the functionality of the LNP-delivered mRNA construct. Expression data was collected after 24hours.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.