GenLNP-S01- Negative Control

GenLNP-S01 Negative Control is an LNP encapsulating non-coding actin RNA with Cap1 and poly(A) tail. Biologically inert, it provides a baseline for RNA delivery and cytotoxicity studies.

价格	￥2000
产品编号	RP-A00078-50
规格	0.05 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The GenLNP-S01 Negative Control contains lipid nanoparticles (LNPs) encapsulating a non-coding actin RNA, designed to serve as a biologically inert control in RNA delivery studies. The LNP is formulated with SM102 as the main ionizable lipid for efficient encapsulation and cellular delivery.

This LNP features:
- Non-coding actin RNA engineered by removing the ATG start codon and downstream initiation signals to prevent protein translation
- Cap1 structure for co-transcriptional capping and a poly(A) tail to mimic mature mRNA stability
- Maintains native RNA sequence composition to minimize nonspecific cellular responses
- Biologically inert design providing high biocompatibility and reliable baseline control

This Negative Control LNP provides a stringent reference for evaluating delivery efficiency and cytotoxicity, enabling clear and reproducible interpretation of experimental results.

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA length	1386 nt
Full mRNA Molecular Weight	446177
Storage buffer	Tris-HCl/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	65-125 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	±15.0 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.