eSpCas9 mRNA (2'-O-ethyl-Cap1, m1Ψ)

eSpCas9 mRNA encodes enhanced-specificity Cas9 with >10-fold reduced off-target effects. Features 2′-O-ethyl-Cap1, m1Ψ modification, and 100A tail. Ideal for precise genome editing and CRISPR research.

价格	￥1500
产品编号	RP-A00074-0.2
规格	0.2 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al., Science (2015 Dec 1). eSpCas9 significantly reduces off-target effects (by more than 10-fold), while maintaining high on-target genome editing efficiency.

This mRNA features:
- A modified 2′-O-ethyl-Cap1 structure with high capping efficiency for enhanced translation and extended intracellular stability
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune activation
- A 100A poly(A) tail to mimic mature mRNA

This construct provides a high-specificity CRISPR editing reagent, ideal for precise genome modification and functional genomics research.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	4479 nt
Full mRNA Molecular Weight	1457890 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect 1 µg of mRNA into one well of cells in a 24-well plate using 1 µL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or an equivalent reagent), following the manufacturer’s instructions. Samples were analyzed at 24, 48, and 72 hours post-transfection using an ELISA assay.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.