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| RP-A00072-0.2 | |
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The eGFP mRNA encodes an enhanced green fluorescent protein (eGFP), with the open reading frame sequence originally derived from the jellyfish Aequorea victoria. eGFP mRNA serves as a widely used fluorescence reporter in mRNA therapeutics, transfection optimization, and delivery system validation. The expressed eGFP protein fluoresces bright green with an excitation wavelength of 488 nm and emission at 507 nm.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 980nt |
| Full mRNA Molecular Weight | 319792 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| 100 ng of mRNA was transfected into 10,000 A549 cells seeded in a 96-well plate using Lipofectamine™ MessengerMAX™ (or an equivalent reagent). The cells were incubated, and expression level was measured at 24, 48, and 72 hours post-transfection using a fluorescent plate reader. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
