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| RP-A00070-0.2 | |
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The Cas13b mRNA encodes Cas13b, an RNA-guided ribonuclease from the type VI CRISPR-Cas system. Cas13b mediates targeted cleavage of single-stranded RNA through complementary binding of a CRISPR RNA (crRNA) spacer to its RNA target, enabling RNA knockdown and transcriptome engineering applications. The sequence of this Cas13b mRNA is referenced from Cox DB, Science, 2017, 358(6366): 1019–1027.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 3586 nt |
| Full mRNA Molecular Weight | 1.16×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| A549 cells were seeded in a 96-well plate at 10,000 cells per well. After overnight incubation, the cells were transfected using Lipofectamine™ MessengerMAX™ (or an equivalent reagent) with 300 ng of Cas13b mRNA, 100 ng of crRNA, and 100 ng of Gluc mRNA per well. The cells were incubated for 24 and 72 hours post-transfection. Three crRNA sequences and one non-targeting control (NTC) were tested. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
