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IL-2 mRNA (Cap1, m1Ψ)

IL-2 mRNA encodes a cytokine that promotes T cell proliferation and NK activity. Features Cap1, m1Ψ modification, 100A tail, and GenScript X4 codon optimization for enhanced expression. Ideal for immunotherapy and immune regulation studies.
¥1500
RP-A00068-0.2

Description

The IL-2 mRNA encodes interleukin-2 (IL-2), a pleiotropic cytokine that promotes T cell proliferation, enhances natural killer (NK) cell cytolytic activity, induces regulatory T cell differentiation, and mediates activation-induced cell death. IL-2 plays a central role in immune regulation and has broad applications in immunotherapy and cell-based research.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency for enhanced translation 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) to improve protein expression and reduce innate immune activation 
- A 100A poly(A) tail to mimic mature mRNA 
- Codon optimization via GenScript X4 algorithm for improved translation efficiency 
 
This IL-2 mRNA enables strong cytokine expression, making it ideal for immune activation studies, T cell biology, and mRNA-based immunotherapy research.

Form Liquid
Concentration 1mg/mL
Full mRNA length 727 nt
Full mRNA Molecular Weight 234414 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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10,000 HEK293T cells were seeded in a 96-well plate on day 0. On the following day, 200 ng of IL-2 mRNA was transfected into the cells using Lipofectamine™ MessengerMAX™ (or an equivalent reagent). The culture supernatant was collected 12 hours post-transfection, and IL-2 levels were measured using a Human IL-2 ELISA kit.
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IL-2 MRNA (Cap1, M1Ψ)

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IL-2 MRNA (Cap1, M1Ψ)

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IL-2 MRNA (Cap1, M1Ψ)

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.