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IL-6 mRNA (Cap1, m1Ψ)

IL-6 mRNA encodes a multifunctional cytokine involved in inflammation and immune regulation. Features Cap1, m1Ψ modification, 100A tail, and GenScript X5 codon optimization for enhanced expression. Ideal for immunology and therapeutic research.
¥1500
RP-A00067-0.2

Description

The IL-6 mRNA encodes interleukin-6 (IL-6), a multifunctional cytokine that acts as both a pro-inflammatory mediator and an anti-inflammatory myokine. IL-6 plays key roles in immune response, inflammation, hematopoiesis, and tissue regeneration, making it a valuable tool in immunology and therapeutic research.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency for enhanced translation 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) to improve protein expression and reduce innate immune activation 
- A 100A poly(A) tail to mimic mature mRNA 
- Codon optimization via GenScript X5 algorithm for enhanced translation efficiency 
 
This IL-6 mRNA enables robust cytokine expression and is ideal for studying inflammatory pathways, immune regulation, and the development of mRNA-based therapeutics.

Form Liquid
Concentration 1mg/mL
Full mRNA length 904 nt
Full mRNA Molecular Weight 292337 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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10,000 HEK293T cells were seeded in a 96-well plate on day 0. On the following day, 200 ng of IL-6 mRNA was transfected into the cells using Lipofectamine™ MessengerMAX™ (or an equivalent reagent). The culture supernatant was collected 12 hours post-transfection, and IL-6 levels were measured using a Human IL-6 ELISA kit.
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IL-6 MRNA (Cap1, M1Ψ)

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IL-6 MRNA (Cap1, M1Ψ)

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IL-6 MRNA (Cap1, M1Ψ)

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.