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| RP-A00066-0.2 | |
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The TNFα mRNA encodes tumor necrosis factor alpha (TNFα), a multifunctional proinflammatory cytokine belonging to the TNF superfamily. TNFα plays a critical role in immune regulation, inflammation, and apoptosis, making it an important molecule for immunology and therapeutic research.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 967 nt |
| Full mRNA Molecular Weight | 312780 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| 10,000 HEK293T cells were seeded in a 96-well plate on day 0. On the following day, 200 ng of TNFα mRNA was transfected into the cells using Lipofectamine™ MessengerMAX™ (or an equivalent reagent). The culture supernatant was collected 12 hours post-transfection, and TNFα levels were measured using a Human TNFα ELISA kit. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
