Cre mRNA V2 (Cap1, m1Ψ)

Cre mRNA V2 encodes P1 bacteriophage Cre recombinase with Cap1, m1Ψ modification, dual NLS, and codon optimization for efficient expression and nuclear targeting. Ideal for gene editing and mammalian cell biology research.

价格	￥1500
产品编号	RP-A00061-0.2
规格	0.2 mg 1 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The Cre mRNA V2 encodes Cre recombinase, a tyrosine recombinase derived from the P1 bacteriophage. The Cre-loxP system is widely used in mammalian genetic studies and cell biology for precise DNA recombination and conditional gene regulation.

This mRNA features:
- A Cap 1 structure with high capping efficiency for enhanced translation
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) to improve protein expression and reduce innate immune activation
- A 100A poly(A) tail to mimic mature mRNA
V2 update:
- Dual NLS at both the 5′ and 3′ ends for efficient nuclear targeting
- Codon optimization via GenScript X5 algorithm for improved expression

Cre mRNA V2 provides robust and reliable recombinase activity, making it ideal for gene editing studies and advanced cell biology applications.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1405 nt
Full mRNA Molecular Weight	455946 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect 0.1 µg of mRNA using 0.5 µL of Lipofectamine™ MessengerMAX™ (or an equivalent reagent) per well of HEK293-LoxP-GFP-RFP(Bsd) cells in a 96-well plate, following the manufacturer’s instructions. RFP expression can be detected using a plate reader with excitation at 560 nm and emission at 620 nm.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.