GS-J2C/CD16a 158V
GS-J2C/CD16A 158V cells overexpressing CD16A 158V and luciferase driven by NFAT RE for application of ADCC Bioassay
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| RD00830 | |
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| Product Description | GS-J2C/CD16A 158V cells overexpressing CD16A 158V and luciferase driven by NFAT RE for application of ADCC Bioassay |
| Culture Properties | Suspension |
| Product Type | Cell line |
| Stability | Stable through more than 25 passages with no significant changes in assay performance or expression profile. |
| Size | 2 vials of frozen cells (>3X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | RPMI 1640, 10% FBS, 1 μg/mL Puromycin, 400 μg/mL Hygromycin B, 800 μg/mL Geneticin |
| Complete Growth Medium | RPMI-1640 with 10% FBS |
| Freeze Medium-DATA | 90% Complete Growth Medium, 10% (V/V) DMSO Or OptiVitro® Cell Cryopreservation Medium |
Flow cytometric analysis of CD16A 158V in GS-J2C/CD16A 158V cells stained with anti-human CD16 Antibody (BioLegend, Cat. No.: 302008) after cell digestion using Accutase Cell Dissociation Reagent. »
Rituxan® (anti-CD20 antibody) were added in the culture of Raji (CD20+) target cells, followed by addition of FcγRIIIa effector cells. After 6-hour incubation, luciferase substrate reagent was added and luminescence signal measured by PHERAstar FSX. Data were analyzed by GraphPad Prism® software. »
Linearity of ADCC reporter assay using Raji as target cells »
Linearity of ADCC reporter assay using Raji as target cells »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
