GS-C4/CGRPR
GS-C4/CGRPR cells overexpress CALCRL, RAMP1 and luciferase driven by cAMP response element function as the effector cells to screen the leads targeting CGRPR or CGRP
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| RD00701 | |
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| 询价 | |
| RD00701 | |
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| Product Description | GS-C4/CGRPR cells overexpress CALCRL, RAMP1 and luciferase driven by cAMP response element function as the effector cells to screen the leads targeting CGRPR or CGRP |
| Culture Properties | Adherent |
| Product Type | Cell line |
| Size | 2 vials of frozen cells (>1X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | F-12K, 10% FBS, 8 μg/mL Puromycin, 400 μg/mL Hygromycin B, 800 μg/mL Geneticin |
| Complete Growth Medium | F-12K, 10% FBS |
| Freeze Medium-DATA | OptiVitro® CD Cell Cryopreservation Medium or 90% Complete Growth Medium, 10% (V/V) DMSO |
GSC4/CGRPR target cells were plated and incubated at 37°C, 5%CO2 for 16-20 hours prior to the addition of increasing concentrations of CGRP. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured by PHERAstar. Data were analyzed by GraphPad Prism® software. »
GS-C4/CGRPR target cells were plated and incubated at 37°C, 5% CO2 for 16-20 hours; then CGRP and increasing concentrations of anti-CGRP antibody were incubated for 30 mins before added to the cells. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured with the PHERAstar. Data were analyzed by GraphPad Prism® software. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
