GSC4/CGRPR target cells were plated and incubated at 37°C, 5%CO₂ for 16-20 hours prior to the addition of increasing concentrations of CGRP. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured by PHERAstar. Data were analyzed by GraphPad Prism® software.

GS-C4/CGRPR target cells were plated and incubated at 37°C, 5% CO₂ for 16-20 hours; then CGRP and increasing concentrations of anti-CGRP antibody were incubated for 30 mins before added to the cells. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured with the PHERAstar. Data were analyzed by GraphPad Prism® software.

GS-C4/CGRPR

GS-C4/CGRPR cells overexpress CALCRL, RAMP1 and luciferase driven by cAMP response element function as the effector cells to screen the leads targeting CGRPR or CGRP

产品编号	RD00701
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	GS-C4/CGRPR cells overexpress CALCRL, RAMP1 and luciferase driven by cAMP response element function as the effector cells to screen the leads targeting CGRPR or CGRP
Culture Properties	Adherent
Product Type	Cell line
Size	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	F-12K, 10% FBS, 8 μg/mL Puromycin, 400 μg/mL Hygromycin B, 800 μg/mL Geneticin
Complete Growth Medium	F-12K, 10% FBS
Freeze Medium-DATA	OptiVitro® CD Cell Cryopreservation Medium or 90% Complete Growth Medium, 10% (V/V) DMSO

图例

GSC4/CGRPR target cells were plated and incubated at 37°C, 5%CO₂ for 16-20 hours prior to the addition of increasing concentrations of CGRP. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured by PHERAstar. Data were analyzed by GraphPad Prism® software.

GS-C4/CGRPR target cells were plated and incubated at 37°C, 5% CO₂ for 16-20 hours; then CGRP and increasing concentrations of anti-CGRP antibody were incubated for 30 mins before added to the cells. After 6 hours of incubation, luciferase substrate reagent was added and luminescence was measured with the PHERAstar. Data were analyzed by GraphPad Prism® software.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

GS-C4/CGRPR

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