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| M01134 | |
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| Product Description | Human Recombinant HEK293/Luc/INSR Stable Cell Line stably overexpress the luciferase reporter (Luc) gene and Insulin receptor (INSR) gene. This stable cell line product is designed for measuring the In Vitro function of INSR or anti-INSR. |
| Culture Properties | Adherent |
| Stability | Stable through more than 20 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen), 6 μg/ml puromycin (Cat. No. A11138-03, Gibco) |
| Complete Growth Medium | DMEM, 10% FBS |
| Freeze Medium-DATA | 42% DMEM (Cat. #10569-010, Gibco), 50% FBS (Cat. No. A5669701, Gibco), 8% DMSO (Cat. No. D2650, Sigma) |
Figure 1: Functional evaluation of HEK293/Luc/INSR cells stimulated by insulin degludec. HEK293/Luc/INSR cells were plated and incubated at 37°C for 16-18 hours prior to the addition of different concentrations of insulin degludec. After 6 hours of incubation, Fire-Lumi™ Reagent (L00877C, GenScript) was added and luminescence measured by PHERAstar.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X) *Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
