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CHO-K1/CRE-CBLuc/GLP1R

Figure 1. GLP-1 (7-37)-induced luciferase expression in CHO-K1/CRE-CBLuc/GLP1R cells. After stimulation by agonist GLP-1 (7-37), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1 (7-37) on cells was 0.12 nM.

CHO-K1/CRE-CBLuc/GLP1R

Recombinant CHO-K1 cells stably overexpress human glucagon like peptide 1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
M00969
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human glucagon like peptide 1 receptor (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham's F-12K (Kaighn's), 10% FBS, 500 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 250 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies).
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma).

图例
  • CHO-K1/CRE-CBLuc/GLP1R
    • CHO-K1/CRE-CBLuc/GLP1R

    Figure 1. GLP-1 (7-37)-induced luciferase expression in CHO-K1/CRE-CBLuc/GLP1R cells. After stimulation by agonist GLP-1 (7-37), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1 (7-37) on cells was 0.12 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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